Improvement of molecular techniques: A multidisciplinar vision


Bruno do Amaral Crispim


The advances in molecular technologies since the discovery of the PCR (Polymerase Chain Reaction) and their
association with the use of molecular markers, allowed a rapid progress in the development of technologies and
equipment able to generate and analyze data on a large scale, revolutionizing research that until recently was only based
on single marker, like the analysis of Single Nucleotide Polymorphism (SNP), and nowadays with the genomic era is
already possible in a few hours genotyping millions or even thousands of SNPs.
This evolution has allowed improvements in research to the knowledge of genomes creating expectations and real
possibilities of application of these techniques in various fields, from medicine to animal production. These new
technologies of molecular analysis of DNA variability determining points of interest in chromosomes, which are
technically called as molecular markers. These markers can be used in various applications, including paternity test,
construction of genetic maps, mapping of quantitative inheritance of characteristics, isolation of genes, marker-assisted
selection and characterization of the genetic diversity of different species.